在S1000 Sephacryl上进行凝胶排阻色谱法：应用于磷脂囊泡

Gel-exclusion chromatography on S1000 Sephacryl: application to phospholipid vesicles.

作者信息

Reynolds J A, Nozaki Y, Tanford C

出版信息

Anal Biochem. 1983 Apr 15;130(2):471-4. doi: 10.1016/0003-2697(83)90618-8.

Abstract

Sephacryl S1000 has an exclusion diameter of approximately 3000 A and is thus an appropriate gel exclusion medium for size analysis and fractionation of phospholipid vesicles. Calibration is conveniently carried out using polystyrene beads of known diameter eluted with a detergent-containing buffer. Recovery of phospholipid vesicles of different sizes from S1000 presaturated with lipid is greater than 95%, and diameters obtained from a calibration curve using the polystyrene beads are in good agreement with those obtained by negative contrast electron microscopy.

摘要

Sephacryl S1000的排阻直径约为3000埃，因此是用于磷脂囊泡大小分析和分级分离的合适凝胶排阻介质。使用已知直径的聚苯乙烯珠在含去污剂的缓冲液中洗脱来方便地进行校准。从预先用脂质饱和的S1000中回收不同大小的磷脂囊泡的回收率大于95%，并且使用聚苯乙烯珠从校准曲线获得的直径与通过负染色电子显微镜获得的直径高度一致。