Skare K L, DeLuca H F
Arch Biochem Biophys. 1983 Jul 1;224(1):13-8. doi: 10.1016/0003-9861(83)90185-6.
Biliary metabolites from physiological doses of all-trans-[10-3H]retinoic acid were examined in normal and vitamin A-deficient rats. The bile from normal and vitamin A-deficient rats contained approximately 60% of the administered dose following a 24-h collection period. However, vitamin A-deficient rats show a 6-h delay in the excretion of radioactivity compared to normal rats. Retinoyl-beta-glucuronide excretion was particularly sensitive to the vitamin A status of the rats. In normal rats, retinoyl-beta-glucuronide reached a maximum concentration of 235 pmol/ml of bile 2 h following the dose and then rapidly declined. Vitamin A-deficient rats show a relatively constant concentration of this metabolite (100-150 pmol/ml of bile) over a 10-h collection period. Retinoic acid excretion was low in both normal and deficient rats. The concentration of retinotaurine, a recently identified biliary metabolite, was approximately equal to retinoyl-beta-glucuronide in normal rats and appeared in the bile 2 h later than the glucuronide.
在正常和维生素A缺乏的大鼠中检测了生理剂量的全反式-[10-³H]视黄酸的胆汁代谢产物。在24小时的收集期后，正常和维生素A缺乏大鼠的胆汁中含有约60%的给药剂量。然而，与正常大鼠相比，维生素A缺乏的大鼠放射性物质的排泄延迟了6小时。视黄酰-β-葡萄糖醛酸苷的排泄对大鼠的维生素A状态特别敏感。在正常大鼠中，给药后2小时视黄酰-β-葡萄糖醛酸苷在胆汁中的浓度达到最高，为235 pmol/ml，然后迅速下降。在10小时的收集期内，维生素A缺乏的大鼠这种代谢产物的浓度相对恒定（胆汁中为100-150 pmol/ml）。正常和缺乏维生素A的大鼠中视黄酸的排泄都很低。一种最近发现的胆汁代谢产物视黄酰牛磺酸的浓度在正常大鼠中与视黄酰-β-葡萄糖醛酸苷大致相等，并且比葡萄糖醛酸苷在胆汁中出现的时间晚2小时。
