Mori T, Yamada Y, Nakajima T, Iwaguchi T
Gan To Kagaku Ryoho. 1983 May;10(5):1342-53.
Effect of tumor burden on the electrophoresis of lymphocytes and macrophages was analyzed with a fully automated cell electrophoretic instrument (Parmoquant). The histogram patterns of peripheral lymphocytes of mice shifted to the lower mobility zone, and that of thymocytes shifted reversely, in the course of development of tumor. In cancer patients, emergence of low mobility T cell (LMT) was observed, and LMT correlated with immunosuppressive factors significantly. The ratio of LMT to high mobility T cells (HMT) tended to increase with tumor growth, and showed extremely high value in the case of recurrent cancers. In the follow-up studies, the LMT/HMT value varied in parallel with tumor growth. The unimodal patterns of macrophages changed to multiple peaks with tumor burden, in experimental animals and in clinical study. At terminal stage, it showed unimodal peak of lower mobility and phagocytic activity decreased. The result indicates that LMT associated with depressed immune state of cancer patients, and electrophoresis of immuno-competent cells may be usefull for cancer diagnosis and early detection of recurrence, because this method is very easy to assay and highly reproducible.
使用全自动细胞电泳仪（Parmoquant）分析肿瘤负荷对淋巴细胞和巨噬细胞电泳的影响。在肿瘤发展过程中，小鼠外周淋巴细胞的直方图模式向低迁移率区域偏移，而胸腺细胞的直方图模式则反向偏移。在癌症患者中，观察到低迁移率T细胞（LMT）的出现，并且LMT与免疫抑制因子显著相关。LMT与高迁移率T细胞（HMT）的比例倾向于随着肿瘤生长而增加，在复发性癌症的情况下显示出极高的值。在随访研究中，LMT/HMT值与肿瘤生长平行变化。在实验动物和临床研究中，巨噬细胞的单峰模式随着肿瘤负荷变为多峰。在末期，它显示出低迁移率的单峰，吞噬活性降低。结果表明，LMT与癌症患者的免疫状态低下相关，并且免疫活性细胞的电泳可能对癌症诊断和复发的早期检测有用，因为该方法易于检测且具有高度可重复性。
