Iwaguchi T
Gan To Kagaku Ryoho. 1986 Mar;13(3 Pt 2):841-8.
The immunological significance of the lymphocyte mobility histogram derived from a fully automated cell electrophoretic instrument (Parmoquant-L), was considered. In mouse lymphocytes, the ratio of medullary thymocytes increased as a result of a decrease in the proportion of cortical thymocytes, whereas the ratio of splenocytes in the low-mobility zone (0.85-0.95 micron/sec/V/cm) increased in the course of tumor growth or in vivo concanavalin A-treatment. In the peripheral lymphocytes of cancer patients, the decreased ratio of high-mobility T cells resulted in a gradual increase in that of low-mobility T cells, which were not seen in lymphocytes modified by the patient's serum. Although the formation of blastoid cells by stimulation of the tumor or mitogen cannot be completely denied, the low-mobility T cells of peripheral lymphoid tissues in the tumor-bearing status are considered to be caused by incomplete differentiation of the cells, in which immunosuppressor cells are involved. Pattern analysis of lymphocyte electrophoresis would be a simple method if fully automated instrumentation was used.
对源自全自动细胞电泳仪（Parmoquant-L）的淋巴细胞迁移率直方图的免疫学意义进行了研究。在小鼠淋巴细胞中，由于皮质胸腺细胞比例下降，髓质胸腺细胞的比例增加，而在肿瘤生长过程中或体内用伴刀豆球蛋白A处理时，低迁移率区（0.85 - 0.95微米/秒/伏/厘米）的脾细胞比例增加。在癌症患者的外周淋巴细胞中，高迁移率T细胞比例下降导致低迁移率T细胞比例逐渐增加，而在患者血清处理的淋巴细胞中未观察到这种情况。尽管不能完全排除肿瘤或有丝分裂原刺激导致类原始细胞形成，但荷瘤状态下外周淋巴组织中的低迁移率T细胞被认为是细胞不完全分化所致，其中涉及免疫抑制细胞。如果使用全自动仪器，淋巴细胞电泳的模式分析将是一种简单的方法。
