Hurley J C, Vadas M A
Aust J Exp Biol Med Sci. 1983 Feb;61(Pt 1):1-9. doi: 10.1038/icb.1983.1.
BALB/c mice develop resistance to challenge with N. dubius third stage infective larvae (L3) 2-3 weeks after the administration of N. dubius worms. In contrast, CBA/H mice fail to develop resistance. Experiments were performed to test the hypothesis that the reason for the different behaviour of these mice was a difference in their eosinophil response. BALB/c mice and BALB/c leads to (BALB/c x CBA/H)F1 bone marrow chimaeras but not CBA/H mice or CBA/H leads to (BALB/c x CBA/H)F1 chimaeras mounted strong eosinophilia after N. dubius sensitization and the eosinophil response was associated with resistance to L3 infection in these mice. Induction of a mild eosinophilia in CBA/H mice by non-parasite antigen keyhole limpet haemocyanin (KLH) in complete Freund's adjuvant (CFA) following cyclophosphamide (CY) treatment was associated with the acquisition of a degree of resistance to L3 infection. In BALB/c mice, increase in eosinophilia induced by CY and KLH-CFA was not associated with a further reduction in worm numbers. These experiments support the hypothesis that eosinophilia is an important component of host protective immunity in vivo, and suggest a new basis for the heterogeneity of the outcome of some parasite infestations.
给BALB/c小鼠接种杜氏小杆线虫后2至3周，它们对杜氏小杆线虫第三期感染性幼虫（L3）的攻击产生抗性。相比之下，CBA/H小鼠未能产生抗性。进行实验以检验以下假设：这些小鼠表现不同的原因在于它们嗜酸性粒细胞反应存在差异。BALB/c小鼠以及BALB/c与（BALB/c×CBA/H）F1骨髓嵌合体小鼠（而非CBA/H小鼠或CBA/H与（BALB/c×CBA/H）F1嵌合体小鼠）在经杜氏小杆线虫致敏后出现强烈的嗜酸性粒细胞增多，且嗜酸性粒细胞反应与这些小鼠对L3感染的抗性相关。在环磷酰胺（CY）处理后，通过在完全弗氏佐剂（CFA）中使用非寄生虫抗原钥孔戚血蓝蛋白（KLH）在CBA/H小鼠中诱导轻度嗜酸性粒细胞增多，与获得一定程度的对L3感染的抗性相关。在BALB/c小鼠中，CY和KLH - CFA诱导的嗜酸性粒细胞增多增加与蠕虫数量的进一步减少无关。这些实验支持了嗜酸性粒细胞增多是体内宿主保护性免疫重要组成部分的假设，并为某些寄生虫感染结果的异质性提出了新的依据。
