Cain K, Skilleter D N
Biochem J. 1983 Mar 15;210(3):769-73. doi: 10.1042/bj2100769.
The time course of cadmium-metallothionein synthesis was studied in non-parenchymal and parenchymal cells, isolated by a cell-separation technique from the livers of rats after the simultaneous injection of CdCl2 (0.05 mg of Cd/kg) and a 10-fold molar excess of 2,3-dimercaptopropanol. Under these conditions of dosing, in contrast with the injection of CdCl2 alone, both cell types accumulate similar concentrations of Cd and synthesize equivalent concentrations of metallothionein. It is concluded that both cell types have a similar capacity to synthesize the metalloprotein, and that the limiting factor under normal cadmium exposure is the relatively inefficient metal uptake into the non-parenchymal cells.
在同时注射氯化镉（0.05毫克镉/千克）和10倍摩尔过量的2,3-二巯基丙醇后，采用细胞分离技术从大鼠肝脏中分离出非实质细胞和实质细胞，研究了镉-金属硫蛋白合成的时间进程。在这些给药条件下，与单独注射氯化镉相比，两种细胞类型积累的镉浓度相似，合成的金属硫蛋白浓度相当。得出的结论是，两种细胞类型合成金属蛋白的能力相似，并且在正常镉暴露情况下，限制因素是金属进入非实质细胞的效率相对较低。
