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核心技术专利：CN118964589B侵权必究

核心技术专利：CN118964589B侵权必究

Grand R J, Perry S V

Biochem J. 1983 Apr 1;211(1):267-72. doi: 10.1042/bj2110267.

A simple method is described for the purification of the alkali and P light chains from chicken gizzard myosin. 2. The sequence of the alkali light chain has been unequivocally determined, except for the N-terminal dipeptide, by using the tryptic and CNBr peptides. 3. No evidence was obtained for any specific high-affinity Ca2+-binding sites on the alkali light chain. 4. Detailed evidence on which the sequence is based has been deposited as Supplementary Publication SUP 50120 (14 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7QB, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1983) 209, 5.

本文描述了一种从鸡胗肌球蛋白中纯化碱性轻链和P轻链的简单方法。2. 除N端二肽外，通过胰蛋白酶肽段和溴化氰肽段已明确确定了碱性轻链的序列。3. 未获得碱性轻链上存在任何特异性高亲和力钙离子结合位点的证据。4. 该序列所依据的详细证据已作为补充出版物SUP 50120（共14页）存放在英国西约克郡韦瑟比市波士顿温泉的大英图书馆文献供应处，其地址为LS23 7QB，可按《生物化学杂志》（1983年）第209卷第5期所示条件从该处获取复印件。

Grand R J, Perry S V

Biochem J. 1983 Apr 1;211(1):267-72. doi: 10.1042/bj2110267.

A simple method is described for the purification of the alkali and P light chains from chicken gizzard myosin. 2. The sequence of the alkali light chain has been unequivocally determined, except for the N-terminal dipeptide, by using the tryptic and CNBr peptides. 3. No evidence was obtained for any specific high-affinity Ca2+-binding sites on the alkali light chain. 4. Detailed evidence on which the sequence is based has been deposited as Supplementary Publication SUP 50120 (14 pages) at the British Library Lending Division, Boston Spa, Wetherby, West Yorkshire LS23 7QB, U.K., from whom copies can be obtained on the terms indicated in Biochem. J. (1983) 209, 5.

本文描述了一种从鸡胗肌球蛋白中纯化碱性轻链和P轻链的简单方法。2. 除N端二肽外，通过胰蛋白酶肽段和溴化氰肽段已明确确定了碱性轻链的序列。3. 未获得碱性轻链上存在任何特异性高亲和力钙离子结合位点的证据。4. 该序列所依据的详细证据已作为补充出版物SUP 50120（共14页）存放在英国西约克郡韦瑟比市波士顿温泉的大英图书馆文献供应处，其地址为LS23 7QB，可按《生物化学杂志》（1983年）第209卷第5期所示条件从该处获取复印件。