Wilkinson J M, Grand R J
Biochem J. 1975 Aug;149(2):493-6.
The complete amino acid sequence of rabbit skeletal muscle troponin I was determined by the isolation of the cyanogen bromide fragments and the tryptic methionine-containing peptides. Troponin I contains 179 amino acid residues and has a molecular weight of 20864. Its N-terminus is acetylated. Detailed evidence on which the sequence is based has been deposited as Supplementary Publication SUP 50055 (23 pages) at the British Library (Lending Division), Boston Spa, Wetherby, West Yorkshire LS23 7QB, U.K., from whom copies may be obtained on the terms given in Biochem. J. (1975) 145, 5.
通过分离溴化氰片段和含甲硫氨酸的胰蛋白酶肽段,确定了兔骨骼肌肌钙蛋白I的完整氨基酸序列。肌钙蛋白I含有179个氨基酸残基,分子量为20864。其N端被乙酰化。该序列所依据的详细证据已作为补充出版物SUP 50055(23页)存放在英国西约克郡韦瑟比波士顿温泉市英国国家图书馆(出借部),邮编LS23 7QB,可按《生物化学杂志》(1975年)第145卷第5期所述条件从该处获取复印件。