二氢蝶啶还原酶作用于[6,6,7,7-2H]7,8(6H)-二氢蝶呤和2,6-二氨基-5-亚氨基嘧啶-4-酮的米氏常数（Km）和催化常数（kcat）值。
Km and kcat. values for [6,6,7,7-2H]7,8(6H)-dihydropterin and 2,6-diamino-5-iminopyrimidin-4-one with dihydropteridine reductase.
作者信息
Armarego W L, Randles D, Taguchi H
出版信息
Biochem J. 1983 May 1;211(2):357-61. doi: 10.1042/bj2110357.
Abstract
The Km and kcat. values for [6,6,7,7-2H]7,8(6H)-dihydropterin and 2,6-diamino-5-iminopyrimidin-4-one were determined for dihydropteridine reductase (EC 1.6.99.10) from two sources. The parameters of the pterin are of the same order as those of the most effective substrates of dihydropteridine reductase. The Km values of the pterin are one order of magnitude smaller than those of the pyrimidinone, although the kcat. values are of the same order.
摘要
测定了来自两个来源的二氢蝶啶还原酶（EC 1.6.99.10）对[6,6,7,7-2H]7,8(6H)-二氢蝶呤和2,6-二氨基-5-亚氨基嘧啶-4-酮的米氏常数（Km）和催化常数（kcat）值。该蝶呤的参数与二氢蝶啶还原酶最有效的底物的参数处于同一数量级。尽管催化常数（kcat）值处于同一数量级，但该蝶呤的米氏常数（Km）值比嘧啶酮的米氏常数小一个数量级。
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