牛精浆主要蛋白质成分PDC-109的一级结构

Primary structure of PDC-109, a major protein constituent of bovine seminal plasma.

作者信息

Esch F S, Ling N C, Böhlen P, Ying S Y, Guillemin R

出版信息

Biochem Biophys Res Commun. 1983 Jun 29;113(3):861-7. doi: 10.1016/0006-291x(83)91078-1.

Abstract

The two major protein components of bovine seminal plasma, PDC-109 and BSP I, have been purified by gel filtration, partition chromatography and reverse-phase high performance liquid chromatography from an 86% ethanol precipitate of bovine seminal plasma ejaculate. The complete 109-residue amino acid sequence of PDC-109 has been established by automated Edman degradation of the intact peptide as well as its proteolytic digestion and cyanogen bromide cleavage fragments. The 12,774 dalton structure has two structurally similar domains of 38 and 41 amino acids, each containing two disulfide bonds.

摘要

牛精浆的两种主要蛋白质成分，即PDC-109和BSP I，已通过凝胶过滤、分配色谱法和反相高效液相色谱法从牛精液射精的86%乙醇沉淀物中纯化出来。通过对完整肽及其蛋白水解消化产物和溴化氰裂解片段进行自动埃德曼降解，已确定了PDC-109完整的109个残基的氨基酸序列。这种12,774道尔顿的结构有两个结构相似的结构域，分别由38个和41个氨基酸组成，每个结构域都含有两个二硫键。