Schonfeld A R, Thal L J, Horowitz S G, Katzman R
Brain Res. 1983 May 5;266(2):271-8. doi: 10.1016/0006-8993(83)90658-3.
Intraseptal administration of heart conditioned medium (HCM) stimulates the growth of injured cholinergic fibers into iris implants placed in the anterodorsal hippocampus. The aims of the present report were to monitor muscarinic cholinergic receptor concentrations during denervation and central innervation of the peripheral tissue targets, and to evaluate the effect of HCM on these changes. For 0, 4, 8, 16 and 28 days after mechanical injury to septohippocampal axons, animals received either intraseptal injections of HCM or control vehicle. Binding of [3H]quinuclidinylbenzilate ([3H]QNB) within iris implants was used as an index of cholinergic muscarinic receptor concentration. The results indicate that within the iris implants: (1) a dramatic drop in the number of muscarinic receptors is observed 4 days after denervation; (2) under control conditions, central cholinergic innervation is not associated with muscarinic receptor recovery; and (3) after administration of HCM, muscarinic receptor levels begin to increase within two weeks and approach the pre-lesion endogenous concentration following 28 days of treatment. These results support the hypothesis that trophic factors may facilitate the restoration of effective, appropriate connections between nerve fibers and their targets.
在中隔内给予心脏条件培养基（HCM）可刺激受损的胆碱能纤维生长至植入前背侧海马体的虹膜组织中。本报告的目的是监测外周组织靶标的去神经支配和中枢神经支配过程中M胆碱能受体的浓度，并评估HCM对这些变化的影响。在海马中隔轴突受到机械损伤后的0、4、8、16和28天，动物接受中隔内注射HCM或对照载体。虹膜组织内[3H]喹核醇基苯甲酸酯（[3H]QNB）的结合被用作胆碱能M受体浓度的指标。结果表明，在虹膜组织内：（1）去神经支配后4天观察到M受体数量急剧下降；（2）在对照条件下，中枢胆碱能神经支配与M受体恢复无关；（3）给予HCM后，M受体水平在两周内开始升高，并在治疗28天后接近损伤前的内源性浓度。这些结果支持了以下假说，即营养因子可能有助于神经纤维与其靶标之间有效、适当连接的恢复。
