Shin H S, McCormick P, Artzt K, Bennett D
Cell. 1983 Jul;33(3):925-9. doi: 10.1016/0092-8674(83)90035-1.
Recessive lethal mutations in the T/t-complex of the mouse characteristically show defective genetic complementation, even when they affect very different stages of embryogenesis and are known to be nonallelic. To address the question of their genetic or functional relationship, we have applied the cis-trans test, using several well defined recombinant t-chromosomes that carry two or more lethal mutations, and others that are devoid of specific lethals. We show here that the defective complementation that occurs between different t-lethals is a specific result of the trans configuration; thus these genes, which may map as much as 15 cM apart, constitute a functional unit. Some speculations are presented to interpret this enigma in terms of DNA plasticity.
小鼠T/t复合体内的隐性致死突变通常表现出遗传互补缺陷，即使它们影响胚胎发育的非常不同阶段且已知是非等位基因。为了解决它们的遗传或功能关系问题，我们应用了顺反测验，使用了几个明确的携带两个或更多致死突变的重组t染色体，以及其他没有特定致死基因的重组t染色体。我们在此表明，不同t致死基因之间出现的互补缺陷是反式构型的特定结果；因此，这些可能相距多达15厘摩的基因构成一个功能单元。我们提出了一些推测，以便从DNA可塑性的角度来解释这一谜团。
