Cheneval D, Müller M, Carafoli E
FEBS Lett. 1983 Aug 8;159(1-2):123-6. doi: 10.1016/0014-5793(83)80429-3.
The phosphate carrier has been isolated from beef heart mitochondria in the presence of cardiolipin and reconstituted in asolectin vesicles. It has been found that 100 microM doxorubicin and 100 microM Br-daunomycin inhibit the unidirectional phosphate uptake in the reconstituted liposomes to the same extent as N-ethylmaleimide. The inhibition by Br-daunomycin is not due to covalent interaction with the carrier. The specific interaction between doxorubicin and cardiolipin is responsible for the inhibition of the phosphate carrier. Br-daunomycin interacts with 3 mitochondrial proteins of apparent Mr approximately 45 000, approximately 35 000 and approximately 30 000.
磷酸载体已在心磷脂存在的情况下从牛心线粒体中分离出来，并重建于大豆卵磷脂囊泡中。已发现100微摩尔的阿霉素和100微摩尔的溴柔红霉素对重建脂质体中单向磷酸盐摄取的抑制程度与N-乙基马来酰亚胺相同。溴柔红霉素的抑制作用并非由于与载体的共价相互作用。阿霉素与心磷脂之间的特异性相互作用导致了对磷酸载体的抑制。溴柔红霉素与三种表观分子量约为45000、约35000和约30000的线粒体蛋白相互作用。
