大鼠血红素结合蛋白-血红素复合物与肝细胞膜受体结合的某些参数的体外研究。
In vitro studies on some parameters of the binding of the rat hemopexin--heme complex with the hepatic membrane receptor.
作者信息
Tran-Quang N, Bernard N, Higa Y, Engler R
出版信息
FEBS Lett. 1983 Aug 8;159(1-2):161-6. doi: 10.1016/0014-5793(83)80438-4.
PMID:6873291
Abstract
The binding of [125I]Hpx--heme with the rat hepatic plasma membrane receptor was studied at 37 degrees C as well as different parameters such as plasma membrane concentration, calcium dependence, optimal pH and specific binding. A Scatchard plot revealed the existence of one binding for [125I]Hpx--heme on the isolated liver plasma membrane with a Kd = 3.2 X 10(-8) M.
摘要
在37℃下研究了[125I]Hpx-血红素与大鼠肝细胞膜受体的结合情况，以及诸如细胞膜浓度、钙依赖性、最佳pH值和特异性结合等不同参数。Scatchard图显示，在分离的肝细胞膜上，[125I]Hpx-血红素存在一种结合，解离常数Kd = 3.2×10^(-8) M。
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