脱氧核糖核酸聚合酶β参与细胞核脱氧核糖核酸的合成。
Involvement of deoxyribonucleic acid polymerase beta in nuclear deoxyribonucleic acid synthesis.
作者信息
Butt T R, Wood W M, McKay E L, Adams R L
出版信息
Biochem J. 1978 Jul 1;173(1):309-14. doi: 10.1042/bj1730309.
The effects on DNA synthesis in vitro in mouse L929-cell nuclei of differential extraction of DNA polymerases alpha and beta were studied. Removal of all measurable DNA polymerase alpha and 20% of DNA polymerase beta leads to a 40% fall in the replicative DNA synthesis. Removal of 70% of DNA polymerase beta inhibits replicative synthesis by 80%. In all cases the nuclear DNA synthesis is sensitive to N-ethylmaleimide and aCTP (arabinosylcytosine triphosphate), though less so than DNA polymerase alpha. Addition of deoxyribonuclease I to the nuclear incubation leads to synthesis of high-molecular-weight DNA in a repair reaction. This occurs equally in nuclei from non-growing or S-phase cells. The former nuclei lack DNA polymerase alpha and the reaction reflects the sensitivity of DNA polymerase beta to inhibiton by N-ethylmaleimide and aCTP.
研究了对小鼠L929细胞核中DNA聚合酶α和β进行差异提取后对体外DNA合成的影响。去除所有可测量的DNA聚合酶α和20%的DNA聚合酶β会导致复制性DNA合成下降40%。去除70%的DNA聚合酶β会使复制性合成受到80%的抑制。在所有情况下，核DNA合成对N-乙基马来酰亚胺和阿糖胞苷三磷酸（aCTP）敏感，尽管比DNA聚合酶α的敏感性要低。向核孵育体系中添加脱氧核糖核酸酶I会在修复反应中导致高分子量DNA的合成。这在非生长细胞或S期细胞的核中均会同样发生。前者的核缺乏DNA聚合酶α，该反应反映了DNA聚合酶β对N-乙基马来酰亚胺和aCTP抑制作用的敏感性。
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