Wist E, Prydz H
Nucleic Acids Res. 1979 Apr;6(4):1583-90. doi: 10.1093/nar/6.4.1583.
The effect of the inhibitor aphidicolin on DNA synthesis in isolated nuclei from HeLa cells and on the activities of partially purified DNA polymerases has been tested. Aphidicolin inhibited DNA synthesis and DNA polymerase alpha very efficiently whereas DNA polymerases beta and gamma were insensitive to the drug. The results indicate that DNA polymerase alpha is the polymerase active during elongation as well as in the gapfilling process of discontinuous DNA synthesis.
已测试了抑制剂阿非迪霉素对HeLa细胞分离细胞核中DNA合成以及部分纯化的DNA聚合酶活性的影响。阿非迪霉素非常有效地抑制了DNA合成和DNA聚合酶α，而DNA聚合酶β和γ对该药物不敏感。结果表明，DNA聚合酶α是在连续DNA合成的延伸过程以及填补缺口过程中具有活性的聚合酶。
