两底物酶促反应中半最大活性底物浓度的扩散性增减
Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions.
作者信息
Engasser J M, Hisland P
出版信息
Biochem J. 1978 Jul 1;173(1):341-3. doi: 10.1042/bj1730341.
Abstract
Diffusional effects on two-substrate enzymic reactions mainly depend on the relative affinities of the enzyme for its two substrates. With two substrates of widely different affinities, diffusional limitations increase and decrease the half-maximal-activity concentration of the high-and low-affinity substrate respectively.
摘要
扩散对双底物酶促反应的影响主要取决于酶对其两种底物的相对亲和力。对于亲和力差异很大的两种底物，扩散限制分别增加和降低高亲和力和低亲和力底物的半数最大活性浓度。
相似文献
1
Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions.两底物酶促反应中半最大活性底物浓度的扩散性增减
Biochem J. 1978 Jul 1;173(1):341-3. doi: 10.1042/bj1730341.
2
Kinetics of soluble and collagen-bound aspartate aminotransferase: diffusional effects with a two-substrate enzymatic reaction.
J Biol Chem. 1977 Nov 25;252(22):7919-22.
3
Diffusional effects on the heterogeneous kinetics of two-substrate enzymic reactions.
J Theor Biol. 1979 Apr 21;77(4):427-40. doi: 10.1016/0022-5193(79)90018-3.
4
A fast evaluation of diffusion effects on bound enzyme activity.对结合酶活性扩散效应的快速评估。
Biochim Biophys Acta. 1978 Oct 12;526(2):301-10. doi: 10.1016/0005-2744(78)90122-5.
5
Steady-state kinetics of ubiquinol-cytochrome c reductase in bovine heart submitochondrial particles: diffusional effects.牛心亚线粒体颗粒中泛醇-细胞色素c还原酶的稳态动力学：扩散效应
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):225-36. doi: 10.1042/bj2900225.
6
The pre-eminence of k(cat) in the manifestation of optimal enzymic activity delineated by using the Briggs-Haldane two-step irreversible kinetic model.通过使用布里格斯 - 霍尔丹两步不可逆动力学模型所描绘的，k(cat)在最佳酶活性表现中的卓越地位。
Biochem J. 1976 Oct 1;159(1):165-6. doi: 10.1042/bj1590165.
7
Determination of intrinsic properties of immobilized enzymes : 2. Kinetic studies on sepharose-staphylococcal nuclease in the presence of diffusional limitations.固定化酶固有性质的测定：2. 在扩散限制存在下琼脂糖-葡萄球菌核酸酶的动力学研究。
Appl Biochem Biotechnol. 1981 Mar;6(1):37-51. doi: 10.1007/BF02779685.
8
[Kinetics model of spherical immobilized cellulase].
Sheng Wu Gong Cheng Xue Bao. 2005 Sep;21(5):799-803.
9
Quantification of kinetics for enzyme-catalysed reactions: implications for diffusional limitations at the 10 ml scale.酶催化反应动力学的量化：10毫升规模下扩散限制的影响。
Biotechnol Lett. 2008 Jun;30(6):995-1000. doi: 10.1007/s10529-008-9645-1. Epub 2008 Jan 26.
10
Influence of substrate and product diffusion on the heterogeneous kinetics of enzymic reversible reactions.
J Theor Biol. 1982 Jan 7;94(1):179-89. doi: 10.1016/0022-5193(82)90339-3.
引用本文的文献
1
Steady-state kinetics of ubiquinol-cytochrome c reductase in bovine heart submitochondrial particles: diffusional effects.牛心亚线粒体颗粒中泛醇-细胞色素c还原酶的稳态动力学：扩散效应
Biochem J. 1993 Feb 15;290 ( Pt 1)(Pt 1):225-36. doi: 10.1042/bj2900225.
2
Role of mobility of redox components in the inner mitochondrial membrane.氧化还原成分在线粒体内膜中的流动性作用。
J Membr Biol. 1988 Sep;104(3):193-209. doi: 10.1007/BF01872322.
本文引用的文献
1
The preparation and kinetics of lactate dehydrogenase attached to water-insoluble particles and sheets.附着于水不溶性颗粒和薄片上的乳酸脱氢酶的制备及其动力学
Biochem J. 1968 Aug;108(5):845-53. doi: 10.1042/bj1080845.
2
Effect of the microenvironment on the mode of action of immobilized enzymes.微环境对固定化酶作用模式的影响。
Adv Enzymol Relat Areas Mol Biol. 1971;34:445-536. doi: 10.1002/9780470122792.ch7.
3
Covalent binding of proteins and glucose-6-phosphate dehydrogenase to cellulosic carriers activated with s-triazine trichloride.蛋白质和葡萄糖-6-磷酸脱氢酶与用三氯均三嗪活化的纤维素载体的共价结合。
Anal Biochem. 1974 Oct;61(2):392-415. doi: 10.1016/0003-2697(74)90406-0.
4
A comparison of some kinetic properties of soluble and bound lactate dehydrogenase isoenzymes at different temperatures.不同温度下可溶性和结合态乳酸脱氢酶同工酶某些动力学特性的比较。
Eur J Biochem. 1976 Aug 1;67(1):87-94. doi: 10.1111/j.1432-1033.1976.tb10636.x.
5
Kinetics of soluble and collagen-bound aspartate aminotransferase: diffusional effects with a two-substrate enzymatic reaction.
J Biol Chem. 1977 Nov 25;252(22):7919-22.
6
Surface-bound aspartate aminotransferase on collagen films. Compared properties with native enzyme.胶原蛋白膜上的表面结合天冬氨酸转氨酶。与天然酶的性能比较。
Biochim Biophys Acta. 1975 Jun 24;391(2):272-81. doi: 10.1016/0005-2744(75)90251-x.
7
Kinetic behavior of immobilized enzyme systems.固定化酶系统的动力学行为
Methods Enzymol. 1976;44:397-443. doi: 10.1016/s0076-6879(76)44031-4.
8
Properties of lactate dehydrogenase immobilized in a lipid-protein matrix.固定在脂蛋白基质中的乳酸脱氢酶的性质。
FEBS Lett. 1976 Dec 15;72(1):93-7. doi: 10.1016/0014-5793(76)80820-4.
Zotero 插件