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两底物酶促反应中半最大活性底物浓度的扩散性增减

Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions.

作者信息

Engasser J M, Hisland P

出版信息

Biochem J. 1978 Jul 1;173(1):341-3. doi: 10.1042/bj1730341.

DOI:10.1042/bj1730341
PMID:687375
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1185780/
Abstract

Diffusional effects on two-substrate enzymic reactions mainly depend on the relative affinities of the enzyme for its two substrates. With two substrates of widely different affinities, diffusional limitations increase and decrease the half-maximal-activity concentration of the high-and low-affinity substrate respectively.

摘要

扩散对双底物酶促反应的影响主要取决于酶对其两种底物的相对亲和力。对于亲和力差异很大的两种底物，扩散限制分别增加和降低高亲和力和低亲和力底物的半数最大活性浓度。

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引用本文的文献

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Steady-state kinetics of ubiquinol-cytochrome c reductase in bovine heart submitochondrial particles: diffusional effects.牛心亚线粒体颗粒中泛醇-细胞色素c还原酶的稳态动力学：扩散效应
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Role of mobility of redox components in the inner mitochondrial membrane.氧化还原成分在线粒体内膜中的流动性作用。
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本文引用的文献

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Biochem J. 1968 Aug;108(5):845-53. doi: 10.1042/bj1080845.
2
Effect of the microenvironment on the mode of action of immobilized enzymes.微环境对固定化酶作用模式的影响。
Adv Enzymol Relat Areas Mol Biol. 1971;34:445-536. doi: 10.1002/9780470122792.ch7.
3
Covalent binding of proteins and glucose-6-phosphate dehydrogenase to cellulosic carriers activated with s-triazine trichloride.蛋白质和葡萄糖-6-磷酸脱氢酶与用三氯均三嗪活化的纤维素载体的共价结合。
Anal Biochem. 1974 Oct;61(2):392-415. doi: 10.1016/0003-2697(74)90406-0.
4
A comparison of some kinetic properties of soluble and bound lactate dehydrogenase isoenzymes at different temperatures.不同温度下可溶性和结合态乳酸脱氢酶同工酶某些动力学特性的比较。
Eur J Biochem. 1976 Aug 1;67(1):87-94. doi: 10.1111/j.1432-1033.1976.tb10636.x.
5
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6
Surface-bound aspartate aminotransferase on collagen films. Compared properties with native enzyme.胶原蛋白膜上的表面结合天冬氨酸转氨酶。与天然酶的性能比较。
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7
Kinetic behavior of immobilized enzyme systems.固定化酶系统的动力学行为
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