附着于水不溶性颗粒和薄片上的乳酸脱氢酶的制备及其动力学
The preparation and kinetics of lactate dehydrogenase attached to water-insoluble particles and sheets.
作者信息
Wilson R J, Kay G, Lilly M D
出版信息
Biochem J. 1968 Aug;108(5):845-53. doi: 10.1042/bj1080845.
Abstract
- The preparation of lactate dehydrogenase covalently attached to anion-exchange cellulose particles and sheets by use of a dichloro-sym-triazinyl dyestuff, Procion brilliant orange MGS, is described. 2. The stability and kinetic properties of these preparations were investigated. 3. An equation is derived to describe the change in concentration of a substrate when passed through a uniform bed of a substrate-inhibited enzyme. A number of theoretical curves are shown to illustrate the system. 4. A titrimetric assay for lactate dehydrogenase is described, and shown to be stoicheiometric over the range pH5.0-9.2. 5. The results are discussed in relation to previous work, and the effects of charged groups on the support, and of the diffusion film surrounding any particle in suspension, are treated qualitatively.
摘要
- 描述了使用二氯 - 对称 - 三嗪基染料Procion亮橙MGS将乳酸脱氢酶共价连接到阴离子交换纤维素颗粒和薄片上的制备方法。2. 研究了这些制剂的稳定性和动力学性质。3. 推导了一个方程来描述底物通过底物抑制酶的均匀床层时浓度的变化。展示了一些理论曲线来说明该系统。4. 描述了一种乳酸脱氢酶的滴定测定法，并表明在pH5.0 - 9.2范围内是化学计量的。5. 结合先前的工作对结果进行了讨论，并定性地探讨了载体上带电基团以及悬浮液中任何颗粒周围扩散膜的影响。
相似文献
1
The preparation and kinetics of lactate dehydrogenase attached to water-insoluble particles and sheets.附着于水不溶性颗粒和薄片上的乳酸脱氢酶的制备及其动力学
Biochem J. 1968 Aug;108(5):845-53. doi: 10.1042/bj1080845.
2
The preparation and properties of pyruvate kinase attached to porous sheets, and the operation of a two-enzyme continuous-feed reactor.附着于多孔片材上的丙酮酸激酶的制备与性质，以及双酶连续进料反应器的运行
Biochem J. 1968 Aug;109(1):137-41. doi: 10.1042/bj1090137.
3
Affinity chromatography of bovine heart lactate dehydrogenase using dye ligands linked directly or spacer-mediated to bead cellulose.使用直接连接或通过间隔臂连接到珠状纤维素上的染料配体对牛心乳酸脱氢酶进行亲和层析。
J Chromatogr. 1989 Apr 19;466:319-29. doi: 10.1016/s0021-9673(01)84627-6.
4
[Inhibition of lactate dehydrogenase by nicotinamide adenine dinucleotide].
Hoppe Seylers Z Physiol Chem. 1968 Mar;349(3):293-6.
5
The chemical attachment of chymotrypsin to water-insoluble polymers using 2-amino-4,6-dichloro-s-triazine.
Biochim Biophys Acta. 1970 Feb 11;198(2):276-85. doi: 10.1016/0005-2744(70)90060-4.
6
A comparison of potato and vertebrate lactate dehydrogenases.马铃薯与脊椎动物乳酸脱氢酶的比较。
Biochem J. 1980 Nov 1;191(2):341-8. doi: 10.1042/bj1910341.
7
The chemical reactivity of the histidine-195 residue in lactate dehydrogenase thiomethylated at the cysteine-165 residue.在半胱氨酸-165残基处硫甲基化的乳酸脱氢酶中组氨酸-195残基的化学反应性。
Biochem J. 1981 Jan 1;193(1):93-7. doi: 10.1042/bj1930093.
8
About the pKa of the active-site histidine in flavocytochrome b2 (yeast L-lactate dehydrogenase).关于黄素细胞色素b2（酵母L-乳酸脱氢酶）活性位点组氨酸的pKa值。
Protein Sci. 1998 Jul;7(7):1531-7. doi: 10.1002/pro.5560070706.
9
10
Difference spectroscopic and kinetic studies on the interaction of lactate dehydrogenase with structurally related triazine dyes.乳酸脱氢酶与结构相关的三嗪染料相互作用的差示光谱和动力学研究。
Biomed Biochim Acta. 1991;50(12):1167-76.
引用本文的文献
1
The preparation and properties of pyruvate kinase attached to porous sheets, and the operation of a two-enzyme continuous-feed reactor.附着于多孔片材上的丙酮酸激酶的制备与性质，以及双酶连续进料反应器的运行
Biochem J. 1968 Aug;109(1):137-41. doi: 10.1042/bj1090137.
2
Studies on the preparation of water-insoluble derivatives of rennin and chymotrypsin and their use in the hydrolysis of casein and the clotting of milk.关于制备凝乳酶和胰凝乳蛋白酶的水不溶性衍生物及其在酪蛋白水解和牛奶凝固中的应用的研究。
Biochem J. 1969 Nov;115(2):183-90. doi: 10.1042/bj1150183.
3
Matrix models. Essential tools for microscopic cytochemical research.矩阵模型。微观细胞化学研究的重要工具。
Histochemistry. 1986;84(4-6):283-300. doi: 10.1007/BF00482953.
4
Diffusional increase and decrease in half-maximal-activity substrate concentrations with two-substrate enzymic reactions.两底物酶促反应中半最大活性底物浓度的扩散性增减
Biochem J. 1978 Jul 1;173(1):341-3. doi: 10.1042/bj1730341.
本文引用的文献
1
Some changes in the reactivity of enzymes resulting from their chemical attachment to water-insoluble derivatives of cellulose.由于酶与不溶于水的纤维素衍生物的化学结合而导致的酶活性的一些变化。
Biochem J. 1968 May;107(5):669-74. doi: 10.1042/bj1070669.
2
Physico-chemical properties of lactic dehydrogenase.乳酸脱氢酶的物理化学性质
J Biol Chem. 1962 Jul;237:2135-9.
3
A WATER-INSOLUBLE POLYANIONIC DERIVATIVE OF TRYPSIN. I. PREPARATION AND PROPERTIES.胰蛋白酶的一种水不溶性聚阴离子衍生物。I. 制备与性质
Biochemistry. 1964 Dec;3:1905-13. doi: 10.1021/bi00900a021.
4
A MICRO-BIURET METHOD FOR ESTIMATING PROTEINS.一种用于蛋白质定量的微量双缩脲法。
Anal Biochem. 1964 Dec;9:401-10. doi: 10.1016/0003-2697(64)90200-3.
5
Preparation and properties of water-insoluble derivatives of trypsin.胰蛋白酶水不溶性衍生物的制备及其性质
J Biol Chem. 1963 May;238:1690-8.
6
Lactic dehydrogenase. IV. The influence of pH on the kinetics of the reaction.乳酸脱氢酶。IV。pH对反应动力学的影响。
J Biol Chem. 1958 Apr;231(2):1065-83.
7
Lactic dehydrogenase. II. Variation of kinetic and equilibrium constants with temperature.乳酸脱氢酶。二。动力学和平衡常数随温度的变化。
J Biol Chem. 1956 Jul;221(1):191-209.
8
Studies on lactic dehydrogenase of heart. I. Purity, kinetics, and equilibria.
J Biol Chem. 1952 Nov;199(1):373-81.
9
Cross-linking of rat tail tendons with chloro-s-triazines.用氯代-s-三嗪对大鼠尾腱进行交联。
Nature. 1967 Jun 24;214(5095):1304-6. doi: 10.1038/2141304a0.
10
Coupling of enzymes to cellulose using chloro-s-triazines.使用氯代-s-三嗪将酶与纤维素偶联。
Nature. 1967 Nov 4;216(5114):514-5. doi: 10.1038/216514a0.
Zotero 插件