Goldberg M, Lelous M, Escaig F, Boudin M
Histochemistry. 1983;78(2):145-56. doi: 10.1007/BF00489493.
Morphologic examination of the developing enamel of rat incisors showed the presence of cell processes and remnants. Histochemical investigation of rapid-frozen freeze-substituted samples, using p-phenylenediamine or a phosphotungstic acid chromic acid mixture, revealed osmiophilic components which were extractable in chloroform-methanol solution and were located inside the tubule-like structures of the extracellular matrix. The presecretory cell-rich and developing enamel zones underwent quantitative and qualitative lipid analysis. Comparison of the biochemical data as well as of the morphological observations, suggests a cellular origin for enamel lipids randomly adsorbed by extracellular matrix components during enamel processing. The nature of the material which appeared as an osmiophilic intra-tubular filling is still unresolved.
对大鼠切牙发育中釉质的形态学检查显示存在细胞突起和残余物。使用对苯二胺或磷钨酸铬酸混合物对快速冷冻的冷冻替代样品进行组织化学研究，发现了亲锇成分，这些成分可在氯仿 - 甲醇溶液中提取，且位于细胞外基质的管状结构内。对富含分泌前期细胞和发育中的釉质区域进行了定量和定性脂质分析。生化数据以及形态学观察结果的比较表明，釉质脂质在釉质形成过程中被细胞外基质成分随机吸附，其来源为细胞。作为亲锇性管内填充物出现的物质的性质仍未明确。
