Moshiri F, Stults L, Novak P, Maier R J
J Bacteriol. 1983 Aug;155(2):926-9. doi: 10.1128/jb.155.2.926-929.1983.
Two H2 uptake-negative (Hup-) Rhizobium japonicum mutants were obtained that also lacked symbiotic N2 fixation (acetylene reduction) activity. One of the mutants formed green nodules and was deficient in heme. Hydrogen oxidation activity in this mutant could be restored by the addition of heme plus ATP to crude extracts. Bacteroid extracts from the other mutant strain lacked hydrogenase activity and activity for both of the nitrogenase component proteins. Hup+ revertants of the mutant strains regained both H2 uptake ability and nitrogenase activity.
获得了两个不能吸收氢气（Hup-）的日本根瘤菌突变体，它们也缺乏共生固氮（乙炔还原）活性。其中一个突变体形成绿色根瘤且血红素缺乏。通过向粗提取物中添加血红素和ATP，该突变体中的氢氧化活性可以恢复。另一个突变菌株的类菌体提取物缺乏氢化酶活性以及两种固氮酶组分蛋白的活性。突变菌株的Hup+回复突变体重新获得了吸收氢气的能力和固氮酶活性。
