游离培养的根瘤菌中氢化酶的测定及大豆根瘤的能量效率。
Determination of Hydrogenase in Free-living Cultures of Rhizobium japonicum and Energy Efficiency of Soybean Nodules.
机构信息
Plant Growth Laboratory, University of California, Davis, California 95616.
出版信息
Plant Physiol. 1978 Oct;62(4):609-11. doi: 10.1104/pp.62.4.609.
Abstract
A sensitive tritium exchange assay was applied to the Rhizobium system for measuring the expression of uptake hydrogenase in free-living cultures of Rhizobium japonicum. Hydrogenase was detected about 45 hours after inoculation of cultures maintained under microaerophilic conditions (about 0.1% O(2)). The tritium exchange assay was used to screen a variety of different strains of R. japonicum (including major production strains) with the findings that about 30% of the strains expressed hydrogenase activity with identical results being observed using an alternative assay based on uptake of H(2). The relative efficiency of intact soybean nodules inoculated with 10 different rhizobial strains gave results identical to those obtained using free-living cultures. The tritium exchange assay provides an easy, quick, and accurate assessment of H(2) uptake efficiency of intact nodules.
摘要
一种灵敏的氚交换测定法被应用于根瘤菌系统，以测量自由生活的日本根瘤菌培养物中摄取氢化酶的表达。在微需氧条件下（约 0.1% O(2)）维持培养物的接种后约 45 小时检测到氢化酶。氚交换测定法用于筛选各种不同的日本根瘤菌菌株（包括主要生产菌株），结果发现约 30%的菌株表达氢化酶活性，使用基于 H(2)摄取的替代测定法观察到相同的结果。用 10 种不同根瘤菌菌株接种完整的大豆根瘤的相对效率与使用自由生活培养物获得的结果相同。氚交换测定法为完整根瘤的 H(2)摄取效率提供了一种简单、快速和准确的评估方法。
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