米索硝唑的分剂量细胞毒性实验。
Split dose cytotoxic experiments with misonidazole.
作者信息
Stratford I J
出版信息
Br J Cancer. 1978 Jul;38(1):130-6. doi: 10.1038/bjc.1978.172.
Abstract
The toxicity of misonidazole (1-(2-nitroimidazol-1-yl)-3-methoxy-2-propanol) towards mammalian cells in vitro has been determined as a function of O2 tension. Misonidazole under hypoxic conditions (less than 10 Parts/10(6) O2) shows the greatest toxicity. Split-dose experiments indicate that lethal damage can be "repaired" by O2, the magnitude of this repair being time dependent and a function of O2 concentration, with maximum repair in air seen after 2 h at 37 degree C. Unlike radiation damage this repair is not inhibited by modest hyperthermia (41 degrees C) during the split-dose interval. The implication of these results as regards the mechanism of misonidazole toxicity under anaerobic conditions is discussed.
摘要
已测定灭滴灵（1-(2-硝基咪唑-1-基)-3-甲氧基-2-丙醇）在体外对哺乳动物细胞的毒性与氧张力的关系。灭滴灵在缺氧条件下（氧含量低于10/10⁶）毒性最大。分次给药实验表明，致死性损伤可被氧“修复”，这种修复的程度与时间有关，且是氧浓度的函数，在37℃下暴露于空气中2小时后修复作用最大。与辐射损伤不同，在分次给药间隔期间，适度的热疗（41℃）不会抑制这种修复。讨论了这些结果对于灭滴灵在厌氧条件下毒性机制的意义。
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本文引用的文献
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Evaluation of a new glutathione-oxidizing reagent for studies of nucleated mammalian cells.
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Mutat Res. 1971 Aug;12(4):453-62. doi: 10.1016/0027-5107(71)90095-9.
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Selective chemotherapy of noncycling cells in an in vitro tumor model.体外肿瘤模型中非循环细胞的选择性化疗。
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