甲硝唑（灭滴灵）和米索硝唑（Ro-07-0582）的细胞毒性：乳酸的增强作用
Cytotoxicity of metronidazole (Flagyl) and misonidazole (Ro-07-0582): enhancement by lactate.
作者信息
Mahood J S, Willson R L
出版信息
Br J Cancer. 1981 Mar;43(3):350-4. doi: 10.1038/bjc.1981.55.
Abstract
The cytotoxic activity of metronidazole (Flagyl) and misonidazole (MISO) to hypoxic Chinese hamster ovary (CHO) cells suspended in standard medium in sealed vials and in gassed spinner flasks has been investigated. Flagyl (5 mM) was only cytotoxic at high initial cell densities. However, when lactate (20 mM) was included in the medium the cytotoxicity of Flagyl at low cell densities was considerable, and similar to that of misonidazole under the same conditions. The relevance of this "lactate effect" to in vivo systems, and the possible mechanisms involved, are discussed.
摘要
已对甲硝唑（灭滴灵）和米索硝唑在密封小瓶及通气搅拌瓶中悬浮于标准培养基的缺氧中国仓鼠卵巢（CHO）细胞的细胞毒性活性进行了研究。灭滴灵（5 mM）仅在高初始细胞密度时具有细胞毒性。然而，当培养基中加入乳酸（20 mM）时，灭滴灵在低细胞密度时的细胞毒性相当大，且与相同条件下米索硝唑的细胞毒性相似。讨论了这种“乳酸效应”与体内系统的相关性以及可能涉及的机制。
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