Greenup C J, Vallera D A, Pennline K J, Kolodziej B J, Dodd M C
Br J Cancer. 1978 Jul;38(1):55-63. doi: 10.1038/bjc.1978.163.
The transfer of tumour-specific cytotoxicity against a murine fibrosar-coma has been demonstrated in vitro using xenogeneic RNA extracted from tumour-cell-immune animals. Poly(A)-tailed messenger RNA from immunogenic RNA was isolated by passage through an oligo(dT)-cellulose column, and evaluated to determine whether the same tumour-specific cytotoxicity could be transferred. Aliquots of normal C3H mouse lymphocytes were treated with poly(A)-containing immune RNA, whole-cell immune RNA lacking poly(A) and total cellular immune RNA. Treated cells were tested in vitro using an adaptation of the Takasugi and Klein microcytotoxicity assay. Percent cytotoxicity was calculted using cells treated with fractions of normal RNA as control. An increase in tumour cytotoxicity was found with poly(A)-containing immune RNA. The optimum dose of poly(A)-tailed immune RNA was estimated as 6.5 microgram of RNA per 4 x 10(6) lymphocytes. Populations of lymphocytes were separated using glass and nylon wool. T- and B-enriched populations were treated with various RNA components. The adherent cell population showed no significant cytotoxicity, whilst treatment of the nonadherent population with poly(A)-tailed immune RNA produced high levels of cytotoxicity.
利用从肿瘤细胞免疫动物中提取的异种RNA，已在体外证明了针对小鼠纤维肉瘤的肿瘤特异性细胞毒性的转移。通过寡聚（dT）-纤维素柱分离来自免疫原性RNA的聚（A）尾信使RNA，并进行评估以确定是否可以转移相同的肿瘤特异性细胞毒性。将正常C3H小鼠淋巴细胞的等分试样用含聚（A）的免疫RNA、缺乏聚（A）的全细胞免疫RNA和总细胞免疫RNA处理。使用Takasugi和Klein微细胞毒性试验的改良方法在体外测试处理过的细胞。使用用正常RNA级分处理的细胞作为对照计算细胞毒性百分比。发现含聚（A）的免疫RNA可增加肿瘤细胞毒性。聚（A）尾免疫RNA的最佳剂量估计为每4×10⁶淋巴细胞6.5微克RNA。使用玻璃和尼龙棉分离淋巴细胞群体。用各种RNA组分处理富含T细胞和B细胞的群体。贴壁细胞群体未显示出明显的细胞毒性，而用聚（A）尾免疫RNA处理非贴壁细胞群体则产生高水平的细胞毒性。
