通过琼脂糖凝胶免疫固定法确认的脑脊液寡克隆区带评估。
An evaluation of cerebrospinal fluid oligoclonal banding confirmed by immunofixation on agarose gel.
作者信息
George P M, Lorier M A, Donaldson I M
出版信息
J Neurol Neurosurg Psychiatry. 1983 Jun;46(6):500-4. doi: 10.1136/jnnp.46.6.500.
Abstract
The cerebrospinal fluid (CSF) from 115 consecutive patients undergoing diagnostic lumbar puncture or myelography was examined to determine the usefulness of immunofixation, following agarose gel electrophoresis, in the detection of oligoclonal IgG. All electrophoretic patterns were evaluated with and without immunofixation, and the interpretation of 9% of specimens was altered by immunofixation. The demonstration of oligoclonal IgG was shown to be more reliable in the diagnosis of multiple sclerosis than other indices of intrathecal synthesis of IgG. It is concluded that immunofixation should be used routinely when examining CSF for oligoclonal banding.
摘要
对115例连续接受诊断性腰椎穿刺或脊髓造影的患者的脑脊液（CSF）进行检查，以确定琼脂糖凝胶电泳后免疫固定法在检测寡克隆IgG中的实用性。所有电泳图谱在有和没有免疫固定的情况下都进行了评估，9%的标本经免疫固定后解释发生了改变。结果表明，寡克隆IgG的显示在多发性硬化症的诊断中比IgG鞘内合成的其他指标更可靠。结论是，在检查脑脊液中的寡克隆带时应常规使用免疫固定法。
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Oligoclonal bands and quantitation of IgG in cerebrospinal fluid as indicators of multiple sclerosis.脑脊液中的寡克隆区带和IgG定量作为多发性硬化症的指标
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Demonstration of cerebrospinal fluid oligoclonal banding in neurologic diseases by agarose gel electrophoresis and immunofixation.通过琼脂糖凝胶电泳和免疫固定法检测神经系统疾病中脑脊液寡克隆区带
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