Kameyama M
J Physiol. 1983 Mar;336:345-57. doi: 10.1113/jphysiol.1983.sp014585.
Guinea-pig hearts were digested enzymatically, resulting in aggregates of cells, cell pairs and single cells. Pairs of cells were chosen for measurement of coupling resistance (rj). Current was injected into one cell of a cell pair. The ratio of the resulting voltage change of the other cell to that of the injected cell was more than 0.8 in most cells (tight electrical coupling) or near 0 in a few cells (functional isolation). rj was affected neither by the transjunctional current, nor by the membrane potential itself. On the other hand, either a reduction of external Na+ concentration (15-60 mM), or an application of ouabain or strophanthidin (10(-5) M) produced a significant increase in rj. It is concluded that low-resistance cell-to-cell junctions are functionally preserved in pairs of dissociated ventricular cells, that the junctional membrane shows no detectable rectification and that intracellular Ca2+ reduces the junctional conductance.
豚鼠心脏经酶消化后，产生细胞聚集体、细胞对和单个细胞。选择细胞对来测量耦联电阻（rj）。向细胞对中的一个细胞注入电流。在大多数细胞中，另一个细胞产生的电压变化与注入细胞的电压变化之比大于0.8（紧密电耦联），而在少数细胞中接近0（功能隔离）。rj既不受跨连接电流的影响，也不受膜电位本身的影响。另一方面，外部Na+浓度降低（15 - 60 mM），或应用哇巴因或毒毛花苷（10(-5) M）都会使rj显著增加。得出的结论是，在分离的心室细胞对中，低电阻细胞间连接在功能上得以保留，连接膜未显示可检测到的整流作用，并且细胞内Ca2+会降低连接电导。
