功能性心脏组织工程学。
Functional cardiac tissue engineering.
机构信息
Department of Biomedical Engineering, Faculty of Cardiology, Duke University, Room 136 Hudson Hall, Durham, NC 27708, USA.
出版信息
Regen Med. 2012 Mar;7(2):187-206. doi: 10.2217/rme.11.122.
Abstract
Heart attack remains the leading cause of death in both men and women worldwide. Stem cell-based therapies, including the use of engineered cardiac tissues, have the potential to treat the massive cell loss and pathological remodeling resulting from heart attack. Specifically, embryonic and induced pluripotent stem cells are a promising source for generation of therapeutically relevant numbers of functional cardiomyocytes and engineering of cardiac tissues in vitro. This review will describe methodologies for successful differentiation of pluripotent stem cells towards the cardiovascular cell lineages as they pertain to the field of cardiac tissue engineering. The emphasis will be placed on comparing the functional maturation in engineered cardiac tissues and developing heart and on methods to quantify cardiac electrical and mechanical function at different spatial scales.
摘要
心脏病发作仍然是全球男性和女性的主要死因。基于干细胞的疗法，包括使用工程化的心脏组织，有可能治疗心脏病发作导致的大量细胞损失和病理性重塑。具体来说，胚胎和诱导多能干细胞是生成治疗相关数量的功能性心肌细胞和体外工程化心脏组织的有前途的来源。本综述将描述与心脏组织工程领域相关的多能干细胞向心血管细胞谱系成功分化的方法。重点将放在比较工程化心脏组织和发育心脏中的功能成熟度上，并介绍在不同空间尺度上量化心脏电和机械功能的方法。
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