Harper A A, Macdonald A G, Wann K T
J Physiol. 1983 May;338:51-60. doi: 10.1113/jphysiol.1983.sp014659.
The action of benzyl alcohol was studied on the voltage-clamped giant axons of Loligo forbesi. The depressant effect of 7.5 mM-benzyl alcohol on the action potential amplitude and peak rate of rise was more marked at a low (8 degrees C) than at a high temperature (16.5 degrees C). A small depolarization (approximately 5 mV) was also produced. These effects were usually reversible. Benzyl alcohol (7.5 mM) selectively depressed the amplitude of the peak early current. The maximum inward current was depressed to 0.65 and 0.67 of the control value at 20 degrees C and 7 degrees C respectively. This effect was usually reversible. Benzyl alcohol also depressed the peak inward conductance (gNa but had no effect on the time to peak early current. A small reversible decrease in the time constant of inactivation (tau h) was caused by benzyl alcohol (7.5 mM). This was usually reversible on washout of the anaesthetic. Benzyl alcohol (7.5 mM) had no effect on the time course of activation or the amplitude of the delayed outward current. The curve of the steady-state inactivation parameter (h infinity) for the Na current against the conditioning membrane potential was shifted by benzyl alcohol in a hyperpolarizing direction (at h infinity = 0.5 the shift was an average of 3.3 mV and was reversible). Increasing the temperature from 7 to 20 degrees C shifted the curve in a depolarizing direction by approximately 10 mV. The reason for the increased nerve-blocking action of benzyl alcohol at the lower temperature is discussed.
研究了苯甲醇对福布斯枪乌贼电压钳制的巨大轴突的作用。7.5 mM苯甲醇对动作电位幅度和峰值上升速率的抑制作用在低温（8℃）时比高温（16.5℃）时更明显。还产生了小的去极化（约5 mV）。这些效应通常是可逆的。7.5 mM苯甲醇选择性地抑制了早期峰值电流的幅度。在20℃和7℃时，最大内向电流分别降至对照值的0.65和0.67。这种效应通常是可逆的。苯甲醇还抑制了峰值内向电导（gNa），但对早期峰值电流的峰值时间没有影响。7.5 mM苯甲醇引起失活时间常数（tau h）的小的可逆性降低。在洗去麻醉剂后，这通常是可逆的。7.5 mM苯甲醇对激活的时间进程或延迟外向电流的幅度没有影响。苯甲醇使钠电流的稳态失活参数（h infinity）曲线相对于调节膜电位向超极化方向移动（在h infinity = 0.5时，移动平均为3.3 mV，且是可逆的）。将温度从7℃升高到20℃使曲线向去极化方向移动约10 mV。讨论了苯甲醇在较低温度下神经阻滞作用增强的原因。
