Bradley D J, Richards C D
Br J Pharmacol. 1984 Jan;81(1):161-7. doi: 10.1111/j.1476-5381.1984.tb10756.x.
The local anaesthetic action of a series of nerve blocking agents was examined at temperatures between 4 degrees C and 20 degrees C in isolated sciatic nerves from cold-adapted frogs. Cooling alone had little effect on the amplitude of the action potential but the conduction velocity was decreased and the duration increased. Cooling had little effect on the local anaesthetic action of the short chain alkanols but that of the long chain alkanols, benzyl alcohol, benzocaine and pentobarbitone was markedly enhanced. The partition of both short and long chain alkanols and of pentobarbitone into a liposome suspension of similar composition to axonal membrane lipids was reduced by cooling from 40 degrees C to 4 degrees C. The results are not compatible with the lipid hypotheses of anaesthetic action. The implications for the nature of the site of action are discussed.
在4摄氏度至20摄氏度的温度范围内，对取自冷适应青蛙的离体坐骨神经，研究了一系列神经阻滞剂的局部麻醉作用。单独冷却对动作电位的幅度影响很小，但传导速度降低，持续时间增加。冷却对短链烷醇的局部麻醉作用影响很小，但对长链烷醇、苯甲醇、苯佐卡因和戊巴比妥的局部麻醉作用有明显增强。从40摄氏度冷却至4摄氏度时，短链和长链烷醇以及戊巴比妥在与轴突膜脂质组成相似的脂质体悬浮液中的分配减少。这些结果与麻醉作用的脂质假说不相符。讨论了对作用部位性质的影响。
