霍乱类毒素诱导的胸腺细胞缺失小鼠中增强的延迟性足垫反应

Augmented delayed footpad reaction in thymus cell-depleted mice induced by cholera toxoid.

作者信息

Tsuru S, Nomoto K, Oka M, Kitani H, Zinnaka Y, Takeya K

出版信息

J Clin Lab Immunol. 1983 May;11(1):37-42.

Abstract

One hundred microgram of cholera toxoid was injected intravenously into DDD and AKR mice and its effects on lymphoid tissues and immune responses against sheep erythrocytes (SRBC) were examined at various times after the injection. (1) A remarkable reduction of thymus cells was revealed from day 1 to 7 and from day 1 to 4 in DDD and AKR mice, respectively. (2) Cholera toxoid exhibited only slight effects on the numbers of spleen cells and peripheral blood leukocytes in both strains. (3) Delayed footpad reactions to SRBC were augmented by a pretreatment with cholera toxoid 4 or 7 days before immunization in both strains. The delayed reactions were not suppressed in the presence of a prominent antibody production and were accompanied by positive macrophage migration inhibition. (4) Antibody production against SRBC, especially of IgG class, was facilitated, when cholera toxoid was given 7 days before the immunization through the footpad in DDD mice. On the other hand, antibody production was suppressed irrespective of immunizing routes and mouse strains, when cholera toxoid was given 1 day before immunization.

摘要

将100微克霍乱类毒素静脉注射到DDD和AKR小鼠体内，并在注射后的不同时间检查其对淋巴组织以及针对绵羊红细胞（SRBC）的免疫反应的影响。（1）分别在DDD和AKR小鼠中，从第1天到第7天以及从第1天到第4天观察到胸腺细胞显著减少。（2）霍乱类毒素对两种品系小鼠的脾细胞和外周血白细胞数量仅产生轻微影响。（3）在两品系小鼠免疫前4天或7天用霍乱类毒素预处理，可增强对SRBC的迟发型足垫反应。在有显著抗体产生的情况下，迟发型反应未被抑制，且伴有阳性巨噬细胞游走抑制。（4）在DDD小鼠中，通过足垫免疫前7天给予霍乱类毒素，可促进针对SRBC的抗体产生，尤其是IgG类抗体。另一方面，当在免疫前1天给予霍乱类毒素时，无论免疫途径和小鼠品系如何，抗体产生均受到抑制。