Mazzorana M, Garrone R
J Submicrosc Cytol. 1983 Jul;15(3):695-703.
The development and substratum adhesion of fresh-water sponges were reversibly altered by penicillamine concentrations of 5 x 10(-3) M to 10(-2) M. Development was delayed and most of the resulting individuals were unable to attach to a substratum. In fixed sponges, the classical lacunose structure was replaced by a dense cellular tissue, imperfectly limited by a discontinuous epithelium. Numerous scattered cells (epithelial and amoeboid) were visible around each sponge. They were either flattened against the glass or in migration. These modifications are interpreted as the result of a penicillamine-induced abnormal synthesis of the collagenous basal layer which normally attaches the whole sponge to its substratum. Due to the loose sponge cell junctions, basal and internal cells could then disaggregate and attach individually to the substratum. Penicillamine treatment could thus be a suitable means of obtaining normal isolated sponge cells and even sponge cell cultures, since classical dissociation of sponge tissue with EDTA gives only rounded cells that are unable to migrate.
浓度为5×10⁻³ M至10⁻² M的青霉胺可使淡水海绵的发育和基质黏附发生可逆性改变。发育延迟，大多数最终形成的个体无法附着于基质。在固定的海绵中，经典的腔隙结构被致密的细胞组织所取代，该组织由不连续的上皮不完全界定。每个海绵周围可见许多散在的细胞（上皮细胞和变形细胞）。它们要么扁平地贴在玻璃上，要么处于迁移状态。这些改变被解释为青霉胺诱导的胶原基底层异常合成的结果，正常情况下该基底层将整个海绵附着于其基质。由于海绵细胞连接松散，基底层细胞和内部细胞随后可能解体并单独附着于基质。因此，青霉胺处理可能是获得正常分离的海绵细胞甚至海绵细胞培养物的合适方法，因为用EDTA对海绵组织进行经典解离只能得到无法迁移的圆形细胞。
