Dufresne-Dube L, Picheral B, Guerrier P
J Ultrastruct Res. 1983 Jun;83(3):242-57. doi: 10.1016/s0022-5320(83)90132-6.
The ultrastructure of Dentalium gametes, and their fate at fertilization, were investigated. Unexpectedly, fixation carried out in 2% osmium tetroxide, at 4 degrees C, although destroying most cytoplasmic organelles, reveals the presence of bundles of "stress fibers" in the microvilli and the fertilization cone of the eggs. Anti-actin labeling supports the view that the bundles are made of actin. Fertilization, as in other molluscs, does not cause any cortical granule exocytosis, at least during the first 15 min following insemination. Ultrastructure of the unreacted and reacted spermatozoon is presented and a tentative model for the morphological interpretation of the acrosomal reaction is proposed. A reevaluation of cytoplasmic heterogeneity, e.g., the so-called cytoplasmic prelocalization, as observed after the germinal vesicle breakdown is also provided as well as an analysis of concomitant surface changes.
研究了象牙贝配子的超微结构及其受精时的命运。出乎意料的是，在4℃下用2%四氧化锇进行固定，尽管破坏了大多数细胞质细胞器，但仍揭示出卵子微绒毛和受精锥中存在“应力纤维”束。抗肌动蛋白标记支持这些束由肌动蛋白组成的观点。与其他软体动物一样，受精至少在授精后的前15分钟内不会引起任何皮质颗粒胞吐作用。展示了未反应和已反应精子的超微结构，并提出了顶体反应形态学解释的初步模型。还提供了对细胞质异质性（例如在生发泡破裂后观察到的所谓细胞质预定位）的重新评估以及伴随的表面变化分析。
