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[用光散射法研究卷曲DNA的动力学]

[Dynamics of coiled DNA by the light scattering method].

作者信息

Ivanova M A, Lomakin A V, Noskin V A

出版信息

Mol Biol (Mosk). 1983 May-Jun;17(3):653-66.

PMID:6877236
Abstract

Quasielastic light scattering investigation of the intrinsic dynamics of the macromolecules in solution is discussed. The character of the spectrum is analysed. The particular case of gaussian chain is discussed in detail. The results of experimental investigation of T7 phage DNA intrinsic dynamics are presented. Due to the regularization procedure it became possible to separate the contributions of pure diffusional and the first relaxation modes. The angular dependence of these modes relaxation time is determined. The results are compared with the Rouse--Zimm theory predictions. It is shown, that the preliminary averaging of the diffusion tensor leads to the qualitative disagreement with the experiment.

摘要

讨论了溶液中大分子本征动力学的准弹性光散射研究。分析了光谱的特征。详细讨论了高斯链的特殊情况。给出了T7噬菌体DNA本征动力学的实验研究结果。由于正则化程序,得以分离出纯扩散模式和第一弛豫模式的贡献。确定了这些模式弛豫时间的角依赖性。将结果与劳斯-齐姆理论预测进行了比较。结果表明,扩散张量的初步平均导致与实验在定性上不一致。

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