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Effects of Na+ on the persistence length and excluded volume of T7 bacteriophage DNA.

作者信息

Sobel E S, Harpst J A

机构信息

Department of Biochemistry, Case Western Reserve University, Cleveland, Ohio 44106-4935.

出版信息

Biopolymers. 1991 Nov;31(13):1559-64. doi: 10.1002/bip.360311311.

DOI:10.1002/bip.360311311
PMID:1814504
Abstract

Total intensity, Rayleigh light scattering has been used to measure the rms radius, second virial coefficient, persistence length, and excluded volume of homogeneous T7 bacteriophage DNA as a function of Na+ concentration (0.005 to 3.0 M). All parameters decrease sharply as [Na+] increases, and tend to level off at high Na+. The variation of persistence length with [Na+] is consistent with predictions from counterion condensation theory.

摘要

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