Rostene W H, Fischette C T, Rainbow T C, McEwen B S
Neurosci Lett. 1983 Jun 16;37(2):143-8. doi: 10.1016/0304-3940(83)90144-1.
Brain sections of 32 microns from the hippocampus were incubated with tritiated serotonin (5-HT) in the presence or absence of 10(-7) M vasoactive intestinal peptide (VIP). Sections were run for biochemical Scatchard analysis or quantitative autoradiography by means of LKB 3H-Ultrofilm. On sections from dorsal hippocampus, VIP increases the amount of 5-HT1 receptors and decreases the affinity for the ligand. Densities measurements show that this effect is located in a discrete area of the hippocampus, the dorsal subiculum. The present data suggest that some of the neurotransmitter effects of 5-HT in the central nervous system can be modulated by VIP.
将取自海马体的32微米脑切片在有或无10⁻⁷ M血管活性肠肽（VIP）的情况下与氚标记的血清素（5-HT）一起孵育。切片用于生化斯卡查德分析或通过LKB ³H - Ultrofilm进行定量放射自显影。在背侧海马体的切片上，VIP增加5-HT1受体的数量并降低对配体的亲和力。密度测量表明这种效应位于海马体的一个离散区域，即背侧下托。目前的数据表明，5-HT在中枢神经系统中的一些神经递质作用可被VIP调节。
