Kiraly M, Audigier S, Tribollet E, Barberis C, Dolivo M, Dreifuss J J
Proc Natl Acad Sci U S A. 1986 Jul;83(14):5335-9. doi: 10.1073/pnas.83.14.5335.
Binding of radioactive vasopressin--but not of oxytocin--was detected by autoradiography and by labeling of membranes obtained from the rat superior cervical ganglion. In both instances binding could be displaced by V1 (smooth muscle-type) but not by V2 (kidney-type) agonists, indicating that the ganglionic vasopressin receptors are similar to those present on hepatocytes and vascular smooth muscle. In accordance with the V1 character of the receptors, vasopressin activated the turnover of membrane inositol lipids, and this effect was abolished by a structural analogue known to act as a vasopressor antagonist. A possible physiological role of vasopressin was suggested by intracellular recordings obtained from ganglion cells in vitro. Vasopressin induced a reduction in the amplitude of the fast excitatory postsynaptic potential evoked by electrical stimulation of the preganglionic nerve. This reduction in ganglionic transmission was antagonized by the same synthetic structural analogue that blocked the effect of vasopressin on inositol lipids. This study provides evidence for the presence of functional vasopressin receptors in a rat sympathetic ganglion and thus suggests that vasopressin may play a role in peripheral autonomic function.
通过放射自显影法以及对取自大鼠颈上神经节的细胞膜进行标记，检测到放射性血管加压素（而非催产素）的结合。在这两种情况下，V1（平滑肌型）激动剂可取代结合，但V2（肾型）激动剂则不能，这表明神经节血管加压素受体与肝细胞和血管平滑肌上的受体相似。根据受体的V1特性，血管加压素激活了膜肌醇脂质的周转，而这种作用被一种已知作为血管升压拮抗剂的结构类似物所消除。体外从神经节细胞获得的细胞内记录提示了血管加压素可能的生理作用。血管加压素可使节前神经电刺激诱发的快速兴奋性突触后电位的幅度降低。神经节传递的这种降低被同一种合成结构类似物所拮抗，该类似物可阻断血管加压素对肌醇脂质的作用。本研究为大鼠交感神经节中存在功能性血管加压素受体提供了证据，因此提示血管加压素可能在外周自主神经功能中发挥作用。
