Brass E P, Forman W B, Edwards R V, Lindan O
Blood. 1978 Oct;52(4):654-8.
Using laser fluctuation spectroscopy, a technique that measures particle size change in solution, the kinetics of fibrin clot formation from fibrinogen can be studied. With this technique the effect of calcium on the three distinguishable phases of clot formation, (1) proteolysis of fibrinogen, (2) fibrinogen-fibrin monomer complex formation, and (3) fibrin monomer polymerization, were investigated. Only a small change in the length of the induction period that results from the fibrinogen-fibrin monomer interactions was observed. However, there was a marked increase in the rate of fibrin monomer polymerization in the presence of calcium ions. These data show that calcium decreases the time required for fibrin formation from fibrinogen by markedly accelerating the phase of fibrin monomer polymerization.
利用激光波动光谱法（一种测量溶液中颗粒大小变化的技术），可以研究由纤维蛋白原形成纤维蛋白凝块的动力学。通过该技术，研究了钙对凝块形成三个可区分阶段的影响，即（1）纤维蛋白原的蛋白水解，（2）纤维蛋白原 - 纤维蛋白单体复合物的形成，以及（3）纤维蛋白单体的聚合。仅观察到由纤维蛋白原 - 纤维蛋白单体相互作用导致的诱导期长度有微小变化。然而，在钙离子存在下，纤维蛋白单体聚合速率显著增加。这些数据表明，钙通过显著加速纤维蛋白单体聚合阶段，减少了从纤维蛋白原形成纤维蛋白所需的时间。
