Perinelli Diego Romano, Bonacucina Giulia, Pucciarelli Stefania, Cespi Marco, Serri Evelina, Polzonetti Valeria, Tambella Adolfo Maria, Vincenzetti Silvia
School of Pharmacy, University of Camerino, 62032 Camerino (MC), Italy.
School of Biosciences and Veterinary Medicine, University of Camerino, 62032 Camerino (MC), Italy.
Biomedicines. 2020 Oct 18;8(10):429. doi: 10.3390/biomedicines8100429.
Platelet-rich plasma (PRP) is a nontransfusional hemocomponent, considered as a powerful concentrate of growth factors (GFs) therapeutically used to stimulate tissue regeneration. The use of autologous PRP, as the patient's own biological material, for therapeutic purposes represents a safe and effective alternative to conventional treatments in both human and veterinary medicine. The aim of this study was the characterization of canine PRP from rheological and biological points of view. Thus, a characterization of the viscoelastic properties of the PRP systems was performed in order to clarify the influence of different calcium concentrations, in the presence of autologous thrombin-rich solution, on the PRP gels' mechanical properties, from which the applicability of these systems in biomedical treatments is strongly dependent. Then, an evaluation of the content of GFs in PRP, activated or not with thrombin, and stored at different temperatures (37 °C and -20 °C) was performed over time, outlining, for the first time, the importance of the effect of physiological temperature (37 °C) on the production of GFs. A clinical case study conducted in a dog with a complete rupture of the common calcaneal tendon (Achilles tendon) confirmed the relevance of this hemocomponent in the daily veterinary clinical activity and the potential translational value for human health.
富血小板血浆（PRP）是一种非输血性血液成分，被认为是一种强大的生长因子（GFs）浓缩物，在治疗上用于刺激组织再生。使用自体PRP作为患者自身的生物材料用于治疗目的，在人类和兽医学中都是传统治疗的一种安全有效的替代方法。本研究的目的是从流变学和生物学角度对犬PRP进行表征。因此，对PRP系统的粘弹性特性进行了表征，以阐明在存在富含自体凝血酶的溶液的情况下，不同钙浓度对PRP凝胶力学性能的影响，而这些系统在生物医学治疗中的适用性强烈依赖于此。然后，对经凝血酶激活或未激活、并在不同温度（37°C和-20°C）下储存的PRP中GFs的含量进行了随时间的评估，首次概述了生理温度（37°C）对GFs产生的影响的重要性。对一只犬跟腱（跟腱）完全断裂的临床病例研究证实了这种血液成分在日常兽医临床活动中的相关性以及对人类健康的潜在转化价值。
