Rogers M A, Marigold J C, Hume S P
Radiat Res. 1983 Jul;95(1):165-74.
The effect of prior administration of retinol, a membrane labilizer, on the in vivo hyperthermal response of lysosomes was investigated in the mouse spleen using a quantitative histochemical assay for the lysosomal enzyme acid phosphatase. A dose of retinol which had no effect when given alone enhanced the thermal response of the lysosome, causing an increase in lysosomal membrane permeability. In contrast, the same dose of retinol had no effect on the gross hyperthermal response of mouse intestine; a tissue which is relatively susceptible to hyperthermia. Thermal damage to intestine was assayed directly by crypt loss 1 day after treatment or assessed as thermal enhancement of X-ray damage by counting crypt microcolonies 4 days after a combined heat and X-ray treatment. Thus, although the hyperthermal response of the lysosome could be enhanced by the administration of retinol, thermal damage at a gross tissue level appeared to be unaffected, suggesting that lysosomal membrane injury is unlikely to be a primary event in hyperthermal cell killing.
使用针对溶酶体酶酸性磷酸酶的定量组织化学分析方法，在小鼠脾脏中研究了预先给予膜稳定剂视黄醇对溶酶体体内热反应的影响。单独给予时无作用的视黄醇剂量增强了溶酶体的热反应，导致溶酶体膜通透性增加。相比之下，相同剂量的视黄醇对小鼠肠道（对热相对敏感的组织）的总体热反应没有影响。治疗后1天通过隐窝损失直接测定肠道的热损伤，或在热和X射线联合治疗后4天通过计数隐窝微菌落评估为X射线损伤的热增强。因此，尽管给予视黄醇可增强溶酶体的热反应，但总体组织水平的热损伤似乎未受影响，这表明溶酶体膜损伤不太可能是热细胞杀伤中的主要事件。
