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[3H-精氨酸、3H-亮氨酸和3H-苯丙氨酸掺入不同倍性肝细胞的强度]

[Intensity of 3H-arginine, 3H-leucine and 3H-phenylalanine incorporation into hepatocytes of various degrees of ploidy].

作者信息

Kudriatsev B N, Maĭtesian E S, Kudriavtseva M V

出版信息

Tsitologiia. 1983 Apr;25(4):441-6.

PMID:6879713
Abstract

The total protein content after Naphtol yellow S staining, the intensity of 3H-amino-acid incorporation, and the DNA content were measured in the same cells on charted preparations of isolated rat hepatocytes. It was shown that the total protein content and intensities of 3H-arginine, 3H-leucine and 3H-phenylalanine incorporation increased proportionally with increasing the cell ploidy. The intensity of aminoacid incorporation on the protein unit did not change in cells of different ploidy, but it decreased in cells of the same polyploidy class, as protein content in cells was increasing.

摘要

在分离的大鼠肝细胞的图谱制剂上,对同一批细胞测量了萘酚黄S染色后的总蛋白含量、³H-氨基酸掺入强度和DNA含量。结果表明,总蛋白含量以及³H-精氨酸、³H-亮氨酸和³H-苯丙氨酸的掺入强度随着细胞倍性的增加而呈比例增加。不同倍性细胞中单位蛋白上氨基酸的掺入强度没有变化,但在同一多倍体类别的细胞中,随着细胞中蛋白质含量的增加,该强度降低。

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1
[Intensity of 3H-arginine, 3H-leucine and 3H-phenylalanine incorporation into hepatocytes of various degrees of ploidy].[3H-精氨酸、3H-亮氨酸和3H-苯丙氨酸掺入不同倍性肝细胞的强度]
Tsitologiia. 1983 Apr;25(4):441-6.
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Tracer methodology in whole body and organ balance metabolic studies: plasma sampling is required. A study in post-absorptive rats using isotopically labeled arginine, phenylalanine, valine and leucine.全身和器官平衡代谢研究中的示踪方法:需要采集血浆样本。一项在禁食大鼠中使用同位素标记的精氨酸、苯丙氨酸、缬氨酸和亮氨酸的研究。
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