Shteĭn G I, Kudriavtsev B N
Tsitologiia. 1988 Dec;30(12):1472-7.
A modified method was proposed for reflected light simultaneous measurement of DNA content and of the silver grain number in the nucleus or cytoplasm of the same cell. Specimens-smears of isolated liver cells incorporated 3H-thymidine and 3H-leucine were prepared on coverslips and after processing were mounted on the slide glasses with smeared side facing downwards to avoid the influence of grains on DNA content measurements. To decrease the background, label measurements were carried out in polarized light. It was shown that the intensity of 3H-leucine incorporation in hepatocytes increases proportionally with cell ploidy degree.
提出了一种改进方法，用于对同一细胞的细胞核或细胞质中的DNA含量和银颗粒数进行反射光同步测量。在盖玻片上制备掺入3H-胸腺嘧啶核苷和3H-亮氨酸的分离肝细胞涂片标本，处理后将涂片面朝下安装在载玻片上，以避免颗粒对DNA含量测量的影响。为了降低背景，在偏振光下进行标记测量。结果表明，肝细胞中3H-亮氨酸的掺入强度与细胞倍性程度成比例增加。
