Bankov N, Karaivanov Kh, Vlakhov K, Petrov M, Kacheva D
Vet Med Nauki. 1983;20(2):6-12.
The possibilities were explored of inducing superovulation and obtaining of zygotes from ewes of the Romanov and Tsigai breeds. The pattern used of inducing superovulation included stimulation with the serum gonadotropin preparation Gravohormone applied at the rates of 750 and 1000 IU, estrus synchronization with Estrumate (ICI, England), and ovulation with the use of the choriogonadotropin preparation Gonabion (Jenapharm, DDR). With Romanov sheep both doses of Gravohormone did not affect essentially the ovarian activity--3.4 and 3.7 ovulations were induced, whereupon a total of 5 (20%) and 10 (38%) ova were obtained from all groups of 7 animals each, 3, resp., 2 only of the zygotes being considered of high quality. With Tsigai sheep a differential ovarian response was induced--3.4 and 6.6 ovulations were produced, whereupon 5 (29%) and 15 (35%) ova were obtained, 2, resp., 7 of them being fertilized, but only 2 of the zygotes being considered fit for transplantation from the group treated with 750 IU of the preparation. No substantial differences were established in the blood plasma estradiol-17 beta and progesterone content and dynamics with the two groups of Romanov sheep treated for superovulation. Results showed that the low response of Romanov sheep, the insufficient fertilization of ova, and the low percent of zygotes capable for transplantation should be further discussed.
对罗曼诺夫和茨盖伊品种母羊进行超数排卵诱导及获取受精卵的可能性进行了探索。超数排卵诱导采用的模式包括以750和1000国际单位的剂量注射血清促性腺激素制剂“孕马血清促性腺激素”（Gravohormone）、用“烯丙孕素”（Estrumate，英国ICI公司生产）进行发情同步化处理以及使用绒促性素制剂“戈那瑞林”（Gonabion，民主德国耶拿制药厂生产）促使排卵。对于罗曼诺夫羊，两种剂量的“孕马血清促性腺激素”对卵巢活动的影响基本不大——分别诱导出3.4次和3.7次排卵，随后从每组7只动物中总共获得了5枚（20%）和10枚（38%）卵子，每组分别只有3枚和2枚受精卵被认为质量较高。对于茨盖伊羊，诱导出了不同的卵巢反应——分别产生3.4次和6.6次排卵，随后获得了5枚（29%）和15枚（35%）卵子，其中分别有2枚和7枚卵子受精，但在接受750国际单位制剂处理的组中，只有2枚受精卵被认为适合移植。对两组接受超数排卵处理的罗曼诺夫羊的血浆雌二醇 - 17β和孕酮含量及动态变化进行比较，未发现显著差异。结果表明，罗曼诺夫羊反应较低、卵子受精不足以及适合移植的受精卵比例较低的问题值得进一步探讨。
