Barth G, Weber H
Z Allg Mikrobiol. 1983;23(3):147-57. doi: 10.1002/jobm.3630230302.
Spontaneous mutants of Saccharomycopsis lipolytica were selected and partially characterized. Several antibiotics and antimetabolites were used for selection of spontaneous resistant mutants from Saccharomycopsis lipolytica. The frequencies of such mutants were mainly arranged between 1 X 10(-7) and 5 X 10(-6) mutants per cell. But one class of glucosamine resistant mutants (GAMRA) occurred more frequently. Among the resistant mutants different types of dominant and recessive resistant mutants could be observed. UV light was used for inactivation of cells and induction of mutants from S. lipolytica. Comparing four haploid strains only small differences were detected in sensitivity to UV light. UV light at a dosage of 135 J/m2 was applied to increase the mutant frequencies in three haploid strains. Besides auxotrophic, temperature sensitive and colony morphology mutants, some new mutant types like small colony forming mutants, red-brown coloured mutants, some new mutant types like small colony forming mutants, red-brown coloured mutants, allylalcohol, glucosamine, 2-deoxyglucose or nystatin resistant mutants, hitherto not described for S. lipolytica, were isolated and partially characterized.
选择了解脂耶氏酵母的自发突变体并对其进行了部分表征。使用了几种抗生素和抗代谢物从解脂耶氏酵母中筛选自发抗性突变体。此类突变体的频率主要在每细胞1×10⁻⁷至5×10⁻⁶个突变体之间。但一类氨基葡萄糖抗性突变体（GAMRA）出现得更为频繁。在抗性突变体中，可以观察到不同类型的显性和隐性抗性突变体。使用紫外线对解脂耶氏酵母细胞进行灭活和诱导突变。比较四个单倍体菌株，在对紫外线的敏感性方面仅检测到微小差异。以135 J/m²的剂量施加紫外线以提高三个单倍体菌株中的突变频率。除了营养缺陷型、温度敏感型和菌落形态突变体外，还分离出了一些新的突变类型，如小菌落形成突变体、红棕色突变体、烯丙醇、氨基葡萄糖、2-脱氧葡萄糖或制霉菌素抗性突变体，这些突变体此前尚未在解脂耶氏酵母中描述过，并对其进行了部分表征。
