Wojtatowicz M, Rymowicz W, Kautola H
Department of Biotechnology and Food Microbiology, Academy of Agriculture, Wroclaw, Poland.
Appl Biochem Biotechnol. 1991 Nov;31(2):165-74. doi: 10.1007/BF02921787.
Four commercial strains and two mutants of the yeast species Yarrowia lipolytica were screened using batch fermentation. Strain Y. lipolytica A-101-1.14 (induced with UV irradiation) was found to be the most suitable for citric acid production from glucose hydrol (39.9% glucose and 2.1% other sugars), a byproduct of glucose production from potato starch. The specific rate of total citric and isocitric acid production was 0.138 g/g.h, the yield on consumed glucose 0.93 g/g, and the productivity achieved was as high as 1.25 g/L.h. All of the tested yeast strains were able to utilize only the glucose from the glucose hydrol medium. Thus, some residual higher oligosaccharides remained in the process effluent.
使用分批发酵法对解脂耶氏酵母的4种商业菌株和2种突变体进行了筛选。发现解脂耶氏酵母A-101-1.14菌株（经紫外线照射诱导）最适合从葡萄糖水解液（39.9%葡萄糖和2.1%其他糖类）中生产柠檬酸，葡萄糖水解液是马铃薯淀粉生产葡萄糖的副产物。柠檬酸和异柠檬酸的总特定生产速率为0.138 g/g·h，消耗葡萄糖的产率为0.93 g/g，实现的生产力高达1.25 g/L·h。所有测试的酵母菌株仅能利用葡萄糖水解液培养基中的葡萄糖。因此，一些残留的高级寡糖仍留在工艺流出物中。
