Sturfelt G, Linder C, Sjöholm A G, Svensson B
Acta Pathol Microbiol Immunol Scand C. 1983 Apr;91(2):151-8.
The influence of serum on the motility of normal monocytes in mononuclear cell preparations was investigated using a modified Boyden assay. Undiluted sera from 9 patients with active SLE and from 6 healthy controls were studied, using pooled normal serum as a reference. When the cells were suspended in SLE serum, both spontaneous motility, and motility in response to zymosan treated reference serum, were reduced. By contrast, the capacity of the SLE sera to generate chemotactic activity on zymosan treatment was hardly affected at all. In zymosan treated control sera, pronounced spreading and decreased motility of monocytes were observed, while zymosan treatment of SLE serum promoted spreading and increased the motility of cells. No consistent relationship was found between complement components and C1q binding immune complexes and the decreased monocyte motility in the SLE sera tested. Cell motility in the assay remained unaffected by the addition of prednisolone to the reference serum.
采用改良的博伊登试验，研究了血清对单核细胞制备物中正常单核细胞运动性的影响。使用混合正常血清作为对照，研究了9例活动期系统性红斑狼疮（SLE）患者和6例健康对照者的未稀释血清。当细胞悬浮于SLE血清中时，自发运动性以及对酵母聚糖处理的对照血清的反应性运动均降低。相比之下，SLE血清经酵母聚糖处理后产生趋化活性的能力几乎未受影响。在酵母聚糖处理的对照血清中，观察到单核细胞明显铺展且运动性降低，而酵母聚糖处理SLE血清则促进铺展并增加细胞运动性。在所检测的SLE血清中，补体成分、C1q结合免疫复合物与单核细胞运动性降低之间未发现一致的相关性。向对照血清中添加泼尼松龙对试验中的细胞运动性没有影响。
