Palleroni N J
Appl Environ Microbiol. 1983 Jun;45(6):1865-9. doi: 10.1128/aem.45.6.1865-1869.1983.
Protoplast formation, fusion, and cell regeneration have been achieved with mutant strains of Actinoplanes brasiliensis. Three-, four-, and five-factor crosses have shown genetic recombination among the markers, and a five-factor cross is analyzed and discussed. Possibilities of using protoplast fusion for gene mapping and strain improvement are suggested.
巴西游动放线菌的突变菌株已实现原生质体的形成、融合及细胞再生。三因子、四因子和五因子杂交显示了标记间的基因重组,并对一次五因子杂交进行了分析和讨论。文中提出了利用原生质体融合进行基因定位和菌株改良的可能性。