Kirichenko N V, Nastasiak I N, Basiliia L I, Fedorenko V A, Danilenko V N
Antibiot Khimioter. 1993 Jan;38(1):24-8.
Formation of genetic recombinants after conjugation and protoplast fusion in Saccharopolyspora erythraea, an organism producing erythromycin, was studied comparatively. After the protoplast fusion the frequency of all the classes of the haploid recombinants increased 10 to 460 times by comparison with the conjugation. The protoplast fusion was characterized by higher diversity of the recombinant classes, up to 45.5 per cent of the recombinants being formed at the account of multiple crossing overs. It was shown that unlike conjugation of the S. erythraea strains the protoplast fusion had no gradient of inheritance of the parent genetic markers by the recombinants. The results indicated that in S. erythraea protoplast fusion the recombination involved more genes of the parent strains. This makes promising the procedure for genetic analysis and design of erythromycin-producing strains.
对红霉素产生菌红多孢菌中接合作用和原生质体融合后遗传重组体的形成进行了比较研究。原生质体融合后，与接合作用相比，所有单倍体重组体类别的频率增加了10至460倍。原生质体融合的特点是重组体类别具有更高的多样性，高达45.5%的重组体是由多次交换形成的。结果表明，与红多孢菌菌株的接合作用不同。原生质体融合中重组体对亲本遗传标记没有遗传梯度。结果表明，在红多孢菌原生质体融合中，重组涉及亲本菌株的更多基因。这使得该程序在红霉素生产菌株的遗传分析和设计方面具有前景。
