Roberts A B, Nichols M D, Frolik C A, Newton D L, Sporn M B
Cancer Res. 1978 Oct;38(10):3327-32.
A separation procedure for retinoids based on reversephase high-pressure liquid chromatography with solvent mixtures of acetonitrile and water is described. The method may be applied to the screening of synthetic retinoids, which have potential for use in the prevention of cancer. It is easily adapted to a variety of biological samples and can be applied to other conventional retinoid assays in liver and plasma, detecting as little as 1 nmol retinyl esters and less than 0.3 nmol retinol per g tissue. The one-step chromatography results in separation and simultaneous determination of many of the synthetic retinoids and all of the natural retinoids, including the retinyl esters that are separated into their major fatty acid components. The method has been applied to the analysis of retinoid levels in the liver and intestine of vitamin A-deficient hamsters following a p.o. dose (0.5 mg/day for 2 days) of retinyl acetate or of a synthetic vitamin A analog and is predictive of the degree to which various synthetic retinoids can be converted to retinol and stored in the liver as retinyl esters. Because of its speed, excellent recoveries, and high resolution, the method offers significant advantages over previous, more lengthy procedures.
描述了一种基于反相高压液相色谱法，使用乙腈和水的混合溶剂分离类视黄醇的方法。该方法可用于筛选具有预防癌症潜力的合成类视黄醇。它易于适用于各种生物样品，并可应用于肝脏和血浆中其他常规类视黄醇测定，每克组织可检测低至1 nmol视黄酯和低于0.3 nmol视黄醇。一步色谱法可分离并同时测定许多合成类视黄醇和所有天然类视黄醇，包括被分离成主要脂肪酸成分的视黄酯。该方法已应用于维生素A缺乏的仓鼠经口给予醋酸视黄酯或合成维生素A类似物（0.5 mg/天，持续2天）后肝脏和肠道中类视黄醇水平的分析，并可预测各种合成类视黄醇转化为视黄醇并作为视黄酯储存在肝脏中的程度。由于其速度快、回收率高和分辨率高，该方法比以前更长的程序具有显著优势。
