Demaugre F, Buc H A, Cepanec C, Moncion A, Leroux J P
Biochem Pharmacol. 1983 Jun 15;32(12):1881-5. doi: 10.1016/0006-2952(83)90053-9.
The respective effects of 2-chloropropionate and dichloroacetate on the pyruvate metabolic crossroads, lipogenesis and ketogenesis, were compared in hepatocytes isolated from fed rats. 2-Chloropropionate acts as an exclusive pyruvate dehydrogenase activator: it increases ketogenesis, lipogenesis, Krebs cycle intermediates and mitochondrial NADH/NAD+ ratio. The effects of dichloroacetate depend on experimental conditions and the intensity of its catabolization into oxalate: the resultant action of dichloroacetate on tested parameters combines the effects of pyruvate dehydrogenase activation on the one hand, and pyruvate carboxylase inhibition by oxalate on the other. A mixture of 2-chloropropionate plus oxalate mimics the effects of dichloroacetate. In hepatocytes from fed rats, endogenous lipogenesis is correlated with the mitochondrial NADH/NAD+ ratio, irrespective of the effector added.
在从喂食大鼠分离出的肝细胞中，比较了2-氯丙酸酯和二氯乙酸对丙酮酸代谢交叉点、脂肪生成和生酮作用的各自影响。2-氯丙酸酯作为唯一的丙酮酸脱氢酶激活剂：它增加生酮作用、脂肪生成、三羧酸循环中间产物以及线粒体NADH/NAD⁺比值。二氯乙酸的作用取决于实验条件及其分解为草酸盐的强度：二氯乙酸对测试参数的最终作用一方面结合了丙酮酸脱氢酶激活的影响，另一方面结合了草酸盐对丙酮酸羧化酶的抑制作用。2-氯丙酸酯加草酸盐的混合物模拟了二氯乙酸的作用。在喂食大鼠的肝细胞中，内源性脂肪生成与线粒体NADH/NAD⁺比值相关，与添加的效应物无关。
