Fesenko E E, Novoselov V I, Krapivinskaya L D, Mjasoedov N F, Zolotarev J A
Biochim Biophys Acta. 1983 Sep 13;759(3):250-6. doi: 10.1016/0304-4165(83)90320-3.
The 25 000 x g pellet of skate and carp olfactory tissue was found to contain structures with high affinity for amino acids (Kd approximately 10(-10) M). Results from competition experiments suggest that a multiplicity of types of olfactory binding sites exists in the skate and carp. Some parameters of a possible receptor for amino acids were evaluated. The isoelectric point of these sites solubilized with Triton X-100 was 5.35 and the molecular weight was about 120 000-150 000.
研究发现，鳐鱼和鲤鱼嗅觉组织经25000倍重力离心后的沉淀中含有对氨基酸具有高亲和力的结构（解离常数约为10⁻¹⁰M）。竞争实验结果表明，鳐鱼和鲤鱼中存在多种类型的嗅觉结合位点。对一种可能的氨基酸受体的一些参数进行了评估。用Triton X-100溶解后的这些位点的等电点为5.35，分子量约为120000 - 150000。
